Microbiology Review Questions

 

Week10ReviewSheet I

 

Exercise1-Disinfectants

 

1.   Whatdoesbactericidalmean?Bacteriostatic?Virucidal?Fungistatic?

 

2.   Whyarecontrolculturesnecessaryinevaluatingdisinfectants?

 

3.   Whatfactorscaninfluencetheactivityofadisinfectant?

 

4.   Whydomicroorganismsdifferintheirresponsetodisinfectants?

 

5.   Whatmicroorganismsaremostsusceptibleto disinfectants?

 

EXERCISE2-AntimicrobialAgentSusceptibilityTestingand Resistance

 

1.   Whatismeantbyantimicrobialresistance?Susceptibility?

 

2.   Whyarepureculturesusedforantimicrobialsusceptibilitytesting?

 

3.   Woulditbeacceptabletouseamixedcultureforthistest?Why?

 

4.   Listthreefactorsthatcaninfluencetheaccuracyofthetest.

 

5.   Whenperformingabrothdilutiontest,whyis itnecessaryto includea growthcontroltube?

 

Asterilitycontroltube?

 

EXERCISE3-TheEnterobacteriaceae(EntericBacilli)

 

 

1.   Whatdoestheterm IMViCmean?

 

2.   Whyis theIMViCusefulinidentifyingEnterobacteriaceae?Arefurtherbiochemicaltests necessaryforcompleteidentification?

 

3.   WhatdiagnostictestdifferentiatesProteusandProvidenciaspeciesfrom other

Enterobacteriaceae?

 

4.   HowisE.colidistinguishedfrom P. vulgarisonMacConkeyagar?OnaTSIslant?

 

5.   Whyisitimportanttodifferentiateglucosenonfermentersfrom Enterobacteriaceae?

 

EXERCISE4–Streptococci,Pneumococci,and Enterococci

 

1.   Differentiatethemicroscopicmorphologyofstreptococciandpneumococciasseenby

 

Gramstain.

 

2.   Whattypeof hemolysisis producedbyS. pneumoniae?

 

3.   HowisS.pneumoniaedistinguishedfrom otherstreptococciwiththesamehemolytic properties?

 

4.   Describethehemolysisproducedbyalpha–hemolytic,beta-hemolytic,andnonhemolytic streptococci.

 

5.   Whattypeofhemolysisis displayedbythegroupablestreptococcithataremostpathogenic forhumanbeings?

 

EXERCISE5-UrineCultureTechniques

 

1.   Whatis bacteriuria?Whenis itsignificant?

 

2.   Howdomicroorganismsentertheurinarytract?

 

3.   Whyisasepticurinecollectionimportantwhenculturesareordered?

 

4.   Listfivebacteriathatcancauseurinarytractinfection.

 

5.   Ifyoucounted20 coloniesfrom a0.01–mlinoculumofa1:10dilutionofurine,howmany organismspermilliliterof specimenwouldyoureport?Isthisnumbersignificant?

 

 

Week10ReviewSheet II

 

EXERCISE1-SerodiagnosisofInfectiousDisease

 

1.   Defineserumtiter.

 

2.   Whatareacuteandconvalescentsera?Whymustbothbetestedtomakeaserological diagnosisof infectiousdisease?

 

3.   Whatis thedifferencebetweenanagglutinationtestanda precipitationtest?

 

4.   Ina pairedserumsample,whattestresultsindicaterecentinfection?

 

5.   Whatis ahumoralantibody?

 

EXERCISE2-CulturingMicroorganismsfromtheEnvironment

 

1.   Whydomicrobiologistswearlaboratorycoats?Didyouconfirmthatthisisnecessary?

 

2.   Whyisitnecessarytowearclean,protectiveclothingwhencaringforapatient?

 

3.   Whyshouldhairbekeptcleanandoutofthewaywhencaringforpatients?

 

4.   Howcanthenumberofmicroorganismsintheenvironmentbecontrolled?

 

5.   Whenandwhyis handwashingimportantinpatientcare?

 

6.   Howcanthosewhocareforpatientsavoidspreadingmicroorganismsamongthem?

 

EXERCISE3-Staphylococci

 

1.   Differentiatethemicroscopicmorphologyofstaphylococciandstreptococciasseenby

Gramstain.

 

2.   Whatarethetwotypesofstaphylococcalcoagulase?

 

3.   Whatis proteinA?Describeonemethodofdetectingit.

 

4.   Whatpropertiesof S.aureusdistinguishitfrom S.epidermidisandS.saprophyticus?

 

5.   Whyarestaphylococcalinfectionsfrequentamonghospitalpatients?

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